Composite

Part:BBa_K2539300:Design

Designed by: Justin Wu   Group: iGEM18_TAS_Taipei   (2018-09-03)


AlcR Expression Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2611
    Illegal BamHI site found at 1615
    Illegal BamHI site found at 2353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1319
    Illegal AgeI site found at 1447
    Illegal AgeI site found at 1978
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

This construct was built to overexpress alcR, a regulatory protein necessary for the activation of PalcA. We acquired all parts from the iGEM distribution kit: a strong promoter and strong RBS combination (BBa_K880005) to maximize protein production, strong RBS (BBa_B0034), alcR (BBa_K2092001), and a double terminator (BBa_B0015) to end transcription.


References